DNA Polymorphism Revealed by Arbitrary Primers Polymerase Chain Reaction Among Blastocystis Strains Isolated from Humans, a Chicken, and a Reptile |
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| Authors: | HISAO YOSHIKAWA ISAO NAGONO EU HIAN YAP MULKIT SINGH YUZO TAKAHASHI |
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| Institution: | Department of Biology, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630, Japan,;Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa-Cho, Gifu 500, Japan, and;Department of Microbiology, Faculty of Medicine, National University of Singapore, Kent Ridge, Singapore 0511, Republic of Singapore |
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| Abstract: | DNA polymorphisms of different strains of Blastocystis isolated from humans, a chicken, and a reptile were examined by an arbitrary primer PCR method. Two strains of Blastocystis hominis isolated from humans in the USA and Japan yielded nearly identical PCR products. However, one strain of B. hominis (isolated from a human in Singapore) yielded quite different PCR products. Blastocystis sp. isolated from a chicken yielded PCR products similar to those of the former two strains, while Blastocystis lapemi, isolated from a reptile, shared no bands with any of the other isolates. These results indicate the possibility that our isolate from the chicken is a zoonotic strain, and that there is intraspecific variation of Blastocystis hominis. |
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| Keywords: | Blastocystis hominis Blastocystis lapemi Blastocystis sp genomic DNA RAPD |
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