| 口蹄疫病毒前导蛋白(Lpro)致牛肾细胞(MDBK)作用的形态学观察 |
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| 引用本文： | 郝峰强,丛国正,高闪电,林彤,独军政,邵军军,常惠芸. 口蹄疫病毒前导蛋白(Lpro)致牛肾细胞(MDBK)作用的形态学观察[J]. 生物工程学报, 2009, 25(11): 1614-1620 |
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| 作者姓名： | 郝峰强 丛国正 高闪电 林彤 独军政 邵军军 常惠芸 |
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| 作者单位： | 1. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,兰州,730046;甘肃农业大学动物医学院,兰州,730070
2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室国家口蹄疫参考实验室,兰州,730046 |
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| 基金项目： | 国家科技支撑计划(No.2006BAD06A14)资助~~ |
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| 摘 要： | 为探讨口蹄疫病毒Lpro致MDBK细胞病变效应中的形态学变化,本实验在成功构建可稳定表达口蹄疫病毒Lpro目的基因的MDBK细胞系的基础上,人工诱导Lpro表达后,采用光学显微镜观察、Hoechst33258染色、AO-EB染色、DNALadder等进行检测,研究口蹄疫病毒Lpro致MDBK细胞的病变效应。结果显示,MDBK细胞系在诱导表达口蹄疫病毒Lpro24h后,光学显微镜下细胞形态表现为细胞体积缩小、核浓缩、细胞周围出现透明圈等现象;Hoechst33258染色检测呈现典型的细胞核浓缩和梅花状核碎裂;诱导表达Lpro36h后,AO-EB染色显示早期病变细胞核染亮绿色呈致密斑块或碎片状,晚期病变细胞核染橘黄色呈致密斑块;DNA凝胶电泳显示可见的DNALadder"梯状"条带。证明口蹄疫病毒Lpro在体外可诱导MDBK细胞发生凋亡。
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| 关 键 词： | 口蹄疫病毒 Lpro MDBK 细胞凋亡 |
| 收稿时间： | 2009-07-16 |
Morphological observation of Bovine Kidney (MDBK) cells effected by foot-and-mouth disease virus Lpro |
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| Fengqiang Hao,Guozheng Cong,Shandian Gao,Tong Lin,Junzheng Du,Junjun Shao and Huiyun Chang. Morphological observation of Bovine Kidney (MDBK) cells effected by foot-and-mouth disease virus Lpro[J]. Chinese journal of biotechnology, 2009, 25(11): 1614-1620 |
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| Authors: | Fengqiang Hao Guozheng Cong Shandian Gao Tong Lin Junzheng Du Junjun Shao Huiyun Chang |
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| Affiliation: | State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China; Animal Medical College, Gansu Agricultural University, Lanzhou 730070, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou 730046, China |
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| Abstract: | In order to explore the morphological changes of Bovine Kidney(MDBK) cells induced by foot-and-mouth disease virus(FMDV) L protease,we induced the expression of FMDV L protease in bovine kidney cells(MDBK) artificially.All work is carried out on the basis of a stable MDBK cell line inducibly expresses the Lab gene under the control of tetracycline.We use cell morphology,Hoechst 33258 staining,AO-EB staining,and DNA Ladder abstraction to research the morphological changes of MDBK cells.24 hours after FMDV L ... |
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| Keywords: | foot-and-mouth disease virus L protease morphological changes of Bovine Kidney apoptosis |
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