Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex

Authors:	Gorynia Sabine Bandeiras Tiago M Pinho Filipa G McVey Colin E Vonrhein Clemens Round Adam Svergun Dmitri I Donner Peter Matias Pedro M Carrondo Maria Arménia

Affiliation:	^aInstituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal;^bBayer Schering Pharma AG, Lead Discovery Berlin – Protein Supply, 13353 Berlin, Germany;^cInstituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal;^dGlobal Phasing Ltd., Sheraton House, Castle Park, Cambridge CB3 0AX, UK;^eEuropean Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, 22603 Hamburg, Germany

Abstract:

Keywords:	Abbreviations: ADP, adenosine diphosphate ATP, adenosine triphosphate BSA, bovine serum albumin dsDNA, double-stranded Deoxyribonucleic acid ssDNA, single-stranded Deoxyribonucleic acid HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid MAD, multi-wavelength anomalous dispersion NCS, non-crystallographic symmetry PEG, polyethylene glycol R1ΔDII, RuvBL1 with truncated domain II R2ΔDII, RuvBL2 with truncated domain II SAXS, small-angle X-ray scattering SeMet, selenomethionine SSM, secondary structure matching SV40 Ltag, Simian Virus 40 large tumor antigen TCEP, tris(2-carboxyethyl)phosphine Tris, tris(hydroxymethyl)aminomethane
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