From 3D cell culture to organs-on-chips |
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Authors: | Huh Dongeun Hamilton Geraldine A Ingber Donald E |
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Affiliation: | 1Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA;2Vascular Biology Program, Departments of Pathology and Surgery, Children's Hospital, Boston and Harvard Medical School, Boston, MA 02115, USA;3School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA |
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Abstract: | 3D cell-culture models have recently garnered great attention because they often promote levels of cell differentiation and tissue organization not possible in conventional 2D culture systems. We review new advances in 3D culture that leverage microfabrication technologies from the microchip industry and microfluidics approaches to create cell-culture microenvironments that both support tissue differentiation and recapitulate the tissue-tissue interfaces, spatiotemporal chemical gradients, and mechanical microenvironments of living organs. These 'organs-on-chips' permit the study of human physiology in an organ-specific context, enable development of novel in vitro disease models, and could potentially serve as replacements for animals used in drug development and toxin testing. |
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