Alterations in protein and gene expression within the barrel cortices of ZnT3 knockout mice: experience-independent and dependent changes |
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Authors: | Nakashima Amy S Butt R Hussain Dyck Richard H |
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Institution: | a Department of Psychology, University of Calgary, Calgary, AB, Canada T2N 1N4 b Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada T2N 4N1 c Department of Cell Biology and Anatomy, University of Calgary, Calgary, AB, Canada T2N 1N4 |
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Abstract: | Much evidence exists for the involvement of vesicular zinc in neurotransmission and cortical plasticity. Recent studies have reported that mice deficient in zinc transporter-3 protein (ZnT3) and thus, vesicular zinc, have significant behavioural and biochemical deficits. Here, we examined whether phenotypic differences existed in the barrel cortices of ZnT3 KO mice using functional proteomics and quantitative PCR. Additionally, by manipulating whisker input, we also investigated experience-dependent changes in protein and gene expression, thereby assaying how cortical plasticity is different in the absence of vesicular zinc. The GABA metabolizing protein ABAT was observed in lower abundances consistently in KO mice. Several presynaptic proteins were identified that were abundant in differing amounts between the WT and KO groups in an experience-dependent manner. At baseline, we observed a decrease in the relative expression of Dlg4, Grin2a, Mt3, and Ntrkb genes in KO mice. The reduced expression of Nrtkb persisted with whisker plucking. These data demonstrate that fundamental changes in the expression of proteins and genes important in neurotransmission occur in the absence of vesicular zinc. Furthermore, the complement of experience-dependent changes were different between WT and KO mice, indicating that the lack of vesicular zinc affects the process of cortical plasticity. |
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Keywords: | CaM calcium/calmodulin CaMKII calcium/calmodulin-dependent protein kinase type II IPG immobilized pH gradient IEF isoelectric focusing KO knockout LS-MS/MS liquid chromatography tandem mass spectrometry MIQE minimum information for publication of quantitative real-time PCR experiments NSF N-ethylmaleimide sensitive fusion protein TrkB neurotrophic tyrosine kinase receptor type 2 NTC no-template controls qPCR quantitative PCR SNARE soluble NSF attachment protein receptor 2DE two-dimensional gel electrophoresis WT wild type ZnT3 or SLC30A3 zinc transporter-3 |
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