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The human ubiquitin C promoter drives selective expression in principal neurons in the brain of a transgenic mouse line |
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| Authors: | Wilhelm Franziska Winkler Ulrike Morawski Markus Jäger Carsten Reinecke Lisa Rossner Moritz J Hirrlinger Petra G Hirrlinger Johannes |
| Affiliation: | a Carl-Ludwig-Institute for Physiology and Interdisciplinary Centre for Clinical Research (IZKF) Leipzig, Faculty of Medicine, University of Leipzig, Liebigstr. 21, D-04103 Leipzig, Germany b Department of Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute of Brain Research, University of Leipzig, Jahnallee 59, D-04109 Leipzig, Germany c Department of Neurogenetics, Max-Planck-Institute of Experimental Medicine, Hermann-Rein-Str. 3, D-37075 Göttingen, Germany d Department of Pathophysiology of Neuroglia, Paul Flechsig Institute of Brain Research, University of Leipzig, Jahnallee 59, D-04109 Leipzig, Germany |
| Abstract: | The specificity of promoters used to drive the expression of proteins of interest is a crucial determinant of transgenesis. Numerous strategies have been developed to restrict expression on a certain cell population. On the other hand it has also remained challenging to obtain ubiquitous expression of transgenes which is needed for example to generate recombination reporter mice or to induce expression by recombination mediated excision of STOP-cassettes. We have generated transgenic mice with the expression of nuclear β-galactosidase driven by the human ubiquitin C promoter thought to mediate ubiquitous expression. However, in the brains of these transgenic mice the expression of the transgene was strikingly limited to principal neurons, while no expression was detected in interneurons or glial cells. These results indicate that the human ubiquitin C promoter might be useful to selectively target projections neurons of the brain. |
| Keywords: | DAB, diaminobenzidine DAPI, 4&prime ,6-diamidino-2-phenylindole FCS, fetal calf serum GOI, gene of interest lacZ, β-galactosidase PBS, phosphate buffered saline PFA, paraformaldehyde UbC, human ubiquitin C X-Gal, bromo-chloro-indolyl-galactopyranoside |
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