Arabidopsis AtIscA-I is affected by deficiency of Fe-S cluster biosynthetic scaffold AtCnfU-V |
| |
Authors: | Yabe Toshiki Nakai Masato |
| |
Institution: | Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita 565-0871, Japan. |
| |
Abstract: | IscA has been proposed to be a scaffold protein of the iron-sulfur cluster biosynthetic machinery. We have identified the IscA homolog to be localized to plastids, termed AtIscA-I, in Arabidopsis thaliana. The AtIscA-I protein was apparently constitutively expressed in all tissues analyzed in Arabidopsis. The AtIscA-I protein exists in the stroma as a soluble protein which tends to form a homo-dimer and can host a 2Fe-2S]-like cluster. Complete loss of the protein from plastids did not cause any significant defect either in normal plant growth or in biogenesis of major iron-sulfur proteins, indicating this protein is not essential or redundant for these functions. In contrast, loss of one of the three plastid-localized CnfU scaffold proteins, AtCnfU-V, caused significant reduction in the level of AtIscA-I. These data suggest that efficient biogenesis of AtIscA-I scaffold requires function of another essential scaffold protein CnfU. |
| |
Keywords: | Iron-sulfur cluster IscA Metal cofactor assembly Chloroplast Ferredoxin CnfU Molecular scaffold Biogenesis Arabidopsis thaliana Plastid |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|