Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats |
| |
Authors: | QianQian Zhao YanLing Feng XiaoFang Jia Lin Yin Ye Zheng DongSheng Ouyang HongHao Zhou LiJun Zhang |
| |
Institution: | 1. Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China 2. Institute of Clinical Pharmacology, Pharmacogenetics Research Institute, Central South University, Changsha, 410078, China
|
| |
Abstract: | Elucidation of the mechanisms of liver fibrogenesis is important to treat liver fibrosis. In this study, we established rat models of liver fibrosis with stages from 0–1, 2, and 3–4 to 4 at 2, 4, 6, and 8 weeks, respectively, by injection of pig serum. Liver fibrogenesis was detected by Masson’s trichrome staining. Rat non-parenchymal cells (NPCs) were enriched 4-fold by Percoll density gradient centrifugation. Protein extracts from NPCs were prepared at 4 and 8 weeks, separated by two-dimensional electrophoresis, and then stained with Coomassie Blue G-250. At 4 weeks, we identified 18 non-redundant differentially expressed proteins of which protein disulfide-isomerase associated protein 3 (PDIA3) and NDUV showed consistent expression at protein and mRNA levels from 4 to 8 weeks. PDIA3 was found to be down-regulated by Western blotting in the rat model and immunohistochemically in human liver. Our results revealed important aspects of the pathogenesis/progression of liver fibrosis and demonstrated important changes in protein expression levels of NPCs at various stages of liver fibrosis. |
| |
Keywords: | liver fibrosis proteomics non-parenchymal cells protein disulfide-isomerase associated protein |
本文献已被 CNKI SpringerLink 等数据库收录! |
|