Rapid degradation of <Emphasis Type="Italic">N</Emphasis>-3-oxo-acylhomoserine lactones by a <Emphasis Type="Italic">Bacillus</Emphasis><Emphasis Type="Italic">cereus</Emphasis> isolate from Malaysian rainforest soil |
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| Authors: | Kok-Gan Chan Cheng-Siang Wong Wai-Fong Yin Choon-Kook Sam Chong-Lek Koh |
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| Institution: | (1) Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;(2) Centre for Research in Biotechnology for Agriculture (CEBAR), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;(3) Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, 637616 Singapore, Singapore |
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| Abstract: | A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically
facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus
cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 μg AHL h−1 per 109 CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif 106HXDH-59 amino acids-H169-21 amino acids-D191 for N-acylhomoserine lactone lactonases. |
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