Molecular cloning of the c2 locus of Zea mays, the gene coding for chalcone synthase |
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Authors: | Udo Wienand, Ulrike Weydemann, Ursula Niesbach-Klö sgen, Peter A. Peterson Heinz Saedler |
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Affiliation: | (1) Max-Planck Institut für Züchtungsforschung, Egelspfad, D-5000 Köln 30, Germany;(2) Department of Agronomy, Iowa State University, 50011 Ames, IA, USA |
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Abstract: | Summary The c2 locus of Zea mays, identified as one of the genes affecting anthocyanin biosynthesis, was cloned using the transposable element En (Spm) as a gene tag. The Spm element present at the c2 locus in the autonomously mutating c2-m1 line was isolated using En1 element specific probes. Sequences flanking the element were identified as c2 locus specific and were used to clone the nonautonomous c2-m2 and wild-type alleles. The cloning and analysis of a cDNA complementary to the c2 locus provided evidence that this gene encodes the enzyme chalcone synthase. |
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Keywords: | Zea mays c2 locus Gene tagging Transposable elements Chalcone synthase |
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