Monitoring the purification by high-performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase-sensitive two-dimensional nuclear magnetic resonance |
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| Authors: | G Otting P Marchot P E Bougis H Rochat K Wüthrich |
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| Affiliation: | Institut für Molekularbiologie und Biophysik, Eidgen?ssiche Technische Hochschule-H?nggerberg, Zürich, Switzerland. |
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| Abstract: | High-resolution phase-sensitive two-dimensional proton nuclear magnetic resonance was used to monitor the preparation by high-performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation VII2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations. |
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