Solubilization and resolution of the membrane-bound nitrite reductase from Paracoccus halodenitrificans into nitrite and nitric oxide reductases |
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Authors: | Grant Michael A Cronin Sonja E Hochstein Lawrence I |
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Institution: | (1) Department of Biology, University of Santa Clara, Santa Clara, CA, USA;(2) Extraterrestrial Research Division, NASA Ames Research Center, 94035 Moffett Field, CA, USA |
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Abstract: | Membranes prepared from Paracoccus halodenitrificans reduced nitrite or nitric oxide to nitrous oxide. Extraction of these membranes with the detergent CHAPSO 3-(3-cholamidopropyldimethylammonio)-1-(2-hydroxy-1-propanesulfonate)], followed by ammonium sulfate fractionation of the solubilized proteins, resulted in the separation of nitrite and nitric oxide reductase activities. The fraction containing nitrite reductase activity spectrally resembled a cd-type cytochrome. Several cytochromes were detected in the nitric oxide reductase fraction. Which, if any, of these cytochromes is associated with the reduction of nitric oxide is not clear at this time.Abbreviations PMS
phenazine methosulfate
- HEPES
N-2-hydroxyethylpiperazine-N -2-ethanesulfonic acid
- CHAPSO
3-(3-cholamidopropyl-dimethylammonio)-1-(2-hydroxy-1-propanesulfonate)
- NH buffer
150 mM NaCl-50 mM
- HEPES
pH 7.5; octylglucoside, octyl- -d glucopyranoside
- NIR
intrite reductase (nitrite to nitric oxide)
- NOR
nitric oxide reductase (nitric oxide to nitrous oxide) |
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Keywords: | Denitrification cd-Cytochrome Nitrite reductase Nitric oxide reductase Paracoccus halodenitrificans |
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