Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells |
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Authors: | Murray Korc Bruce E. Magun |
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Affiliation: | 1. Department of Medicine University of Arizona Health Sciences Center Tucson, Arizona 85724, USA;1. Department of Anatomy University of Arizona Health Sciences Center Tucson, Arizona 85724, USA |
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Abstract: | The binding of 125I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture. |
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