Tissue distribution of cytochrome c oxidase isoforms in mammals. Characterization with monoclonal and polyclonal antibodies |
| |
Authors: | Jan-Willem Taanaman Robin E Hall Chunlin Tang Michael F Marusich Nancy G Kennaway Roderick A Capaldi |
| |
Institution: | 1. Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA;2. Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, OR 97201, USA |
| |
Abstract: | Monoclonal and polyclonal antibodies specific to the two isoforms of subunit VIa of bovine cytochrome c oxidase were generated and used to study the tissue distribution of this subunit pair in beef, human and rat. The so-called H-(heart) form was found exclusively in heart and skeletal muscle, whereas the so-called L-(liver) form was the only isoform present in brain, kidney, liver and smooth muscle. Little or no L-form was detected in skeletal muscle. In bovine heart no subunit VIa-L was detected, while in human heart the subunit VIa-H and VIa-L isoforms were present in roughly equal proportions. These results imply that, in humans, the deficiency of a subunit VIa isoform may have a different effect on the physiology of heart then on the physiology of skeletal muscle. |
| |
Keywords: | Mitochondrial protein Tissue-specific gene expression Monoclonal antibody (Rat) (Beef) (Human) BSA bovine serum albumin CFA complete Freund's adjuvant DTT dithiothreitol FPBS PBS with 2% fetal bovine serum IFA incomplete Freund's adjuvant PMSF phenylmethylsulfonyl fluoride PVDF polyvinylidine difluoride |
本文献已被 ScienceDirect 等数据库收录! |
|