Isoenzyme-specific quantitative immunoassays for cytosolic glutathione transferases and measurement of the enzymes in blood plasma from cancer patients and in tumor cell lines |
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Authors: | Xiao-Yong Hao Victor M Castro Jonas Bergh Birgitta Sundström Bengt mannervik |
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Institution: | 1. Department of Biochemistry, Biomedical Center, Uppsala University, Uppsala, Sweden;2. Department of Oncology, Akademiska Sjukhuset, Uppsala University, Uppsala, Sweden;3. Sangtec Medical AB, Stockholm, Sweden |
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Abstract: | Enzyme-linked immunoassays (ELISAs) based on the double-antibody sandwich technique have been developed for the quantitative analysis of the major human cytosolic class Pi, Mu and Alpha glutathione transferases (GSTs). The procedures were optimized with respect to antibody concentration for coating of plates as well as other parameters in order to achieve high sensitivity and accuracy. No cross-reactivity was detected between members of the three different classes of GSTs or among the Mu class GSTs M2-2, M3-3 and M4-4 with the ELISA for GST M1-1. The ELISAs have been applied to establish the cytosolic GST profiles of 10 cell lines and to monitor the plasma GST levels in cancer patients. The results revealed that the class Pi GST was the dominant isoenzyme in six (LS 174T, HCT-8, Hu 549 Pat, K-562, U-937 and Hu 549) out of nine tumor cell lines and immortalized hepatocytes (Chang Liver). The isoenzymes A1-1 and M1-1 were determined to be the major GST components in Hep G2 and HeLa cells, respectively. In a clinical study, the majority of the patients with urinary bladder cancer were found to have increased plasma levels of both GST A1-1 and GST P1-1 (10/15), while patients with renal cancer frequently showed increases only in GST P1-1 (5/8). The results demonstrate that the ELISAs are suitable for analyzing GST phenotypes in both normal and tumor cells and in monitoring plasma levels of GSTs in cancer patients. |
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Keywords: | Glutathione transferase Tumor cell line Enzyme-linked immunosorbent assay Blood plasma Ab Anitbody Ag Antigen BSA bovine serum albumin ELISA enzyme-linked immunosorbent assay GST glutathione trasnferase Ig G immunoglobulin G PBS phophate-buffered saline PF4 platelet factor 4 |
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