| 红树DNA的十六烷基三甲基溴化铵法提取及其随机扩增多态DNA反应 |
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| 引用本文： | 吴多桂,林栖凤,李冠一.红树DNA的十六烷基三甲基溴化铵法提取及其随机扩增多态DNA反应[J].中国生物化学与分子生物学报,1999,15(1):67-70. |
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| 作者姓名： | 吴多桂 林栖凤 李冠一 |
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| 作者单位： | 海南大学生物科学技术研究所 |
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| 基金项目： | 国家计委“九五”重点科技攻关项目 |
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| 摘 要： | 采用ＣＴＡＢ法提取了耐盐植物红树ＤＮＡ，所得ＤＮＡ样品的紫外吸收Ａ２５８／Ａ２８０比值为１．８９，纯度能符合限制性内切酶酶切、ＰＣＲ反应以及ＤＮＡ重组克隆等分子生物学操作的要求．方法简便，容易掌握，较普通的酚－氯仿法有明显的优点．还探讨了以ＣＴＡＢ法制备的红树ＤＮＡ为模板进行ＲＡＰＤ反应参数的优化组合
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| 关 键 词： | 红树 DNA 十六烷基三甲基溴化铵 随机扩增多态DNA |
| 收稿时间： | 1999-02-20 |
Cetyl trimethyl Ammonium Bromide(CTAB)Method for Extracting Rhizophora apiculata's DNA and its Random Amplified Polymorphic DNA(RAPD)Reaction |
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| WU Duogui,LIN Qifeng,LI Guanyi.Cetyl trimethyl Ammonium Bromide(CTAB)Method for Extracting Rhizophora apiculata's DNA and its Random Amplified Polymorphic DNA(RAPD)Reaction[J].Chinese Journal of Biochemistry and Molecular Biology,1999,15(1):67-70. |
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| Authors: | WU Duogui LIN Qifeng LI Guanyi |
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| Institution: | (Institute of Biological Science and Technology,Hainan University,Haikou 570228 |
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| Abstract: | An easily manipulated CTAB method was used to extract DNA from Rhizophora apiculata ,one kind of the salt resistant plants.The A 258 / A 280 value of DNA extracted was 1 89,which was suitable for many manipulations in molecular biology such as restriction endonuclease digestion,polymerase chain reaction and the DNA's recombination and cloning.Reaction conditions for RAPD technique were also optimized.The RAPD reaction of 35 cycles resulted in the same as that of 45 cycles and one hour or so was saved. |
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| Keywords: | Rhizophora apiculata DNA Cetyltrimethyl ammonium bromide(CTAB) Radom amplified polymorphic DNA(RAPD) |
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