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Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model |
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| Authors: | Alibaud Laeticia Köhler Thilo Coudray Alice Prigent-Combaret Claire Bergeret Evelyne Perrin Jackie Benghezal Mohammed Reimmann Cornelia Gauthier Yves van Delden Christian Attree Ina Fauvarque Marie-Odile Cosson Pierre |
| Affiliation: | Département de Physiologie Cellulaire et Métabolisme and;Département de Microbiologie et de Médecine Moléculaire, Universitéde Genève, Centre Médical Universitaire, 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland.; Service des Maladies Infectieuses, Hôpital Universitaire de Genève, Rue Micheli-du-Crest 24, CH-1211 Geneva 14, Switzerland.; CEA, DSV, iRTSV/LTS, Laboratoire de Transduction du Signal, 38054 Grenoble, France;INSERM, U873, France;UniversitéJoseph Fourier, France.; Département de Microbiologie Fondamentale, Universitéde Lausanne, CH-1015 Lausanne, Switzerland.; Département de Biologie des Agents Transmissibles, Centre de Recherche du Service de Santédes Armées, 24 Avenue des Maquis du Grésivaudan, BP 87, 38702 La Tronche Cedex, France.; CEA, iRTSV/LBBSI, CNRS, UJF, 38054, France. |
| Abstract: | The human pathogen Pseudomonas aeruginosa has been shown previously to use similar virulence factors when infecting mammalian hosts or Dictyostelium amoebae. Here we randomly mutagenized a clinical isolate of P. aeruginosa , and identified mutants with attenuated virulence towards Dictyostelium . These mutant strains also exhibited a strong decrease in virulence when infecting Drosophila and mice, confirming that P. aeruginosa makes use of similar virulence traits to confront these very different hosts. Further characterization of these bacterial mutants showed that TrpD is important for the induction of the quorum-sensing circuit, while PchH and PchI are involved in the induction of the type III secretion system. These results demonstrate the usefulness and the relevance of the Dictyostelium host model to identify and analyse new virulence genes in P. aeruginosa . |
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