Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity |
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| Authors: | B L Slomiany J Bilski J Sarosiek V L Murty B Dworkin K VanHorn J Zielenski A Slomiany |
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| Institution: | 1. Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea;2. Department of Biochemistry, College of Oriental Medicine, Dong-Eui University, Busan47340, Republic of Korea;1. Institute of Food Science Research, CIAL (CSIC-UAM), Nicolás Cabrera, 9, 28049 Madrid, Spain;2. Hospital La Paz, Health Research Institute (IdiPaz), Paseo de la Castellana, 261, 28046 Madrid, Spain;3. IMDEA-Food Institute, CEI (UAM+CSIC), Carretera de Canto Blanco, 8, 28049 Madrid, Spain;1. Department of Pathophysiology, Shantou University Medical College, 5150412, People’s Republic of China;2. Shenzhen University General Hospital, 518055, People’s Republic of China;3. Department of Dermatology, First Affiliated Hospital, Shantou University Medical College, 515041, People’s Republic of China;4. Huizhou Municipal Hospital, People’s Republic of China;1. Biogeochemistry Research Group, Geography Department, Trinity College Dublin, Dublin 2, Ireland;2. GeoZentrum Nordbayern (GZN), Universität Erlangen-Nürnberg, Loewenichstr. 28, 91054 Erlangen, Germany;1. Department of Psychology, Virginia State University, United States;2. VCU Pauley Heart Center, Virginia Commonwealth University, United States;3. Institute for Drug and Alcohol Studies, Virginia Commonwealth University, United States;4. University of Florida Center for AIDS/HIV Research, Service, and Education University of Florida, United States;1. Department of Life and Nanopharmaceutical Sciences, Graduate school, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea;2. Department of Pharmacy, College of Pharmacy, Kyung Hee University, 26 Kyungheedae-ro, Dozgdaemun-gu, Seoul 02447, Republic of Korea;3. Department of Oriental Pharmaceutical Science, College of Pharmacy and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea |
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| Abstract: | The role of Campylobacter pyloridis, a spiral bacteria associated with gastritis and peptic ulcers in weakening the mucus component of gastric mucosal barrier was investigated. The colonies of bacteria, cultured from antral mucosal biopsies of patients undergoing gastroscopy, were washed with saline, passed through sterilization filter and the filtrate was examined for protease and glycosylhydrolase activities. The obtained results revealed that the filtrate exhibited a strong proteolytic activity not only towards the typical protein substrates such as albumin but also towards gastric mucin. Optimum enzymatic activity for degradation of mucin was attained at pH 7.0 and the protease activity was found in a low m.w. (less than 50K) protein fraction. The filtrate showed little glycosylhydrolase activity and did not cause the hydrolysis of mucin carbohydrates. The data suggest that C pyloridis infection weakens the gastric mucosal defense by causing proteolytic degradation of mucin component of the protective mucus layer. |
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