Addition of allochthonous fungi to a historically contaminated soil affects both remediation efficiency and bacterial diversity |
| |
Authors: | Ermanno Federici Vanessa Leonardi Maria A Giubilei Daniele Quaratino Roberta Spaccapelo Alessandro D’Annibale Maurizio Petruccioli |
| |
Institution: | (1) Dipartimento di Medicina Sperimentale e Scienze Biochimiche, University of Perugia, Perugia, Italy;(2) Dipartimento di Agrobiologia e Agrochimica, Università degli Studi della Tuscia, Via S. C. De Lellis, 01100 Viterbo, Italy;(3) Interuniversitary National Consortium “The Chemistry for the Environment”, Marghera, Venice, Italy |
| |
Abstract: | Botryosphaeria rhodina DABAC P82 and Pleurotus pulmonarius CBS 664.97 were tested for their ability to grow and to degrade aromatic hydrocarbons in an aged contaminated soil. To evaluate
the impact of indigenous microflora on the overall process, incubations were performed on both fumigated and nonfumigated
soils. Fungal colonization by B. rhodina was unexpectedly lower in the fumigated than in the nonfumigated soil while the growth of P. pulmonarius showed an opposite response. Degradation performances and detoxification by both fungi in the nonfumigated soil were markedly
higher than those observed in the fumigated one. Heterotrophic bacterial counts in nonfumigated soil augmented with either
B. rhodina or P. pulmonarius were significantly higher than those of the corresponding incubation control (6.7 ± 0.3 × 108 and 8.35 ± 0.6 × 108, respectively, vs 9.2 ± 0.3 × 107). Bacterial communities of both incubation controls and fungal-augmented soil were compared by numerical analysis of denaturing
gradient gel electrophoresis profiles of polymerase chain reaction (PCR)-amplified 16S ribosomal RNA (rRNA) genes and cloning
and sequencing of PCR-amplified 16S rRNA genes. Besides increasing overall diversity, fungal augmentation led to considerable
qualitative differences with respect to the pristine soil. |
| |
Keywords: | Mycoremediation Bioaugmentation Historically contaminated soil DGGE Microbial interactions Aromatic hydrocarbons |
本文献已被 PubMed SpringerLink 等数据库收录! |
|