An assat for PEP carboxykinase in crude tissue extracts |
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Authors: | M D Hatch |
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Institution: | Division of Plant Industry, Commonwealth Scientific Industrial and Research Organization, Canberra City, A.C.T. 2601, Australia |
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Abstract: | A simple procedure is described for assaying phosphoenolpyruvate carboxykinase in the direction of oxaloacetate decarboxylation by following the decrease in oxaloacetate absorbance. The procedure offers a means of measuring the activity of this enzyme in its physiological direction in unfractionated tissue extracts. |
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