Confirmation of the role of auxin and calcium in the late phases of adventitious root formation |
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| Authors: | Jalil Bellamine Claude Penel Hubert Greppin Thomas Gaspar |
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| Affiliation: | (1) Institute of Botany B 22, University of Liege, Sart Tilman, B –, 4000 Liège, Belgium;(2) Present address: Laboratory of Plant Physiology and Biochemistry, University of Geneva, CH –, 1211 Genève 4, Switzerland |
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| Abstract: | Poplar shoots raised in vitro were induced to root by incubation on an auxin (NAA) containing medium for 7 h. After 13 days on an auxin-free medium, 97% of the treated shoots had rooted. The introduction of known antiauxins (PCIB, PBA, POAA) into the rooting expression auxin-free medium, after the 7-h induction by NAA, completely (PCIB and PBA) or severely (POAA) inhibited rooting. The exclusion of calcium from the expression auxin free medium reduced the percentage of rooting by about 42%. The inhibition was still higher in the presence of EGTA, a calcium chelator. Lanthanum chloride, a calcium channel blocker, also completely inhibited rooting, when incorporated into the auxin free medium, with or without calcium. These results support previous hypotheses about the implication of both endogenous auxin and calcium in the late phases of the adventitious rooting process. |
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| Keywords: | adventitious rooting anti-auxins lanthanum chloride poplar shoots |
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