Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling |
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Authors: | K Hayashi J Kawase K Yoshimura K Ara K Tsuji |
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Affiliation: | Tochigi Research Laboratories, Kao Soap Company, Ltd., 2606, Akabane, Ichikai-machi, Tochigi, Japan |
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Abstract: | A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 micrograms/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water. |
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Keywords: | fatty acid metal salt HPLC microdetermination fluorescence detection prederivatization |
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