REGULATION OF OXYGEN CONSUMPTION IN NEUROBLASTOMA CELLS: EFFECTS OF DIFFERENTIATION AND OF POTASSIUM |
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| Authors: | C Nissen J Ciesielski-Treska L Hertz P Mandel |
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| Institution: | Centre de Neurochimie du CNRS, Strasbourg, France1;Department of Biochemistry A, University of Copenhagen, Denmark2 |
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| Abstract: | Abstract— The rate of oxygen consumption has been measured micromanometrically in fresh mouse neuroblastoma cells and in corresponding cells cultivated with and without 20% calf serum known to suppress differentiation. Fresh cells and cells cultivated in the serum depleted medium showed a relatively intense respiration (0.5–1.0 × 10−5μl/h/cell), whereas the proliferating, cultivated cells had a low rate of oxygen uptake (0.2 × 10-5μl/h/cell), provided they did not differentiate morphologically during the 4 h of measurement in the serum-free diver medium. Both morphological and metabolic differentiation of such cells occurs rapidly but may be completely prevented by siliconization of the divers.
An increase of the potassium concentration in the medium to 50 mM had essentially no effect on cultivated, differentiated cells, but led to an abolishment of oxygen uptake by fresh cells. No stimulatory effect of potassium was observed. |
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