Sequence and evolution of the Drosophila pseudoobscura glycerol-3-phosphate dehydrogenase locus |
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Authors: | R. Spencer Wells |
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Affiliation: | (1) Museum of Comparative Zoology Laboratories, Harvard University, 02138 Cambridge, MA, USA;(2) Present address: Department of Structural Biology, Stanford University School of Medicine, 94305 Stanford, CA, USA |
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Abstract: | The Gpdh genomic region has been cloned and sequenced in Drosophila pseudoobscura. A total of 6.8 kb of sequence was obtained, encompassing all eight exons of the gene. The exons have been aligned with the sequence from D. melanogaster, and the rates of synonymous and nonsynonymous substitution have been compared to those of other genes sequenced in these two species. Gpdh has the lowest rate of nonsynonymous substitution yet seen in genes sequenced in both D. pseudoobscura and D. melanogaster. No insertion/deletion events were observed, and the overall architecture of the gene (i.e., intron sites, etc.) is conserved. An interesting amino acid reversal was noted between the D. melanogaster Fast allele and the D. pseudoobscura gene. |
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Keywords: | Glycerol-3-phosphate dehydrogenase Gpdh Drosophila pseudoobscura Homoplasy Allozyme |
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