Differentiated inhibition of DNA, RNA and protein synthesis in L1210 cells by 8-methoxypsoralen |
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Authors: | P E Nielsen W P Linnane |
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Institution: | Department of Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3 C, DK-2200 Copenhagen, Denmark |
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Abstract: | The synthesis of DNA, RNA and protein was measured in L1210 cells following treatment with 8-methoxypsoralen in combination with long wavelength ultraviolet irradiation. The results show that the DNA synthesis is strongly inhibited (approximately 95%) at 200 ng/ml reaching a minimum within 2 hours while RNA synthesis is only weakly affected at this concentration (approximately 40% inhibition). At 2 micrograms/ml the RNA synthesis is inhibited approximately 90%. Even at this concentration only a moderate effect is seen on the protein synthesis. These results strongly indicate that the phototoxic action of 8-methoxypsoralen is primarily due to inhibition of DNA synthesis. |
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Keywords: | 8MOP 8-methoxypsoralen PBS phosphate buffered saline TCA trichloro-acetic acid |
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