Enhancement of tryptophan production by Escherichia coli as an application of genetic engineering |
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Authors: | Shuichi Aiba Tadayuki Imanaka Hiroshi Tsunekawa |
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Affiliation: | (1) Department of Fermentation Technology, Faculty of Engineering, Osaka Univ., Yamada-kami, Suita-shi, Osaka, Japan |
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Abstract: | Summary A feedback resistant trp operon plasmid that transformed a multiple mutant (trpR tnaA) of Escherichia coli was found to enhance remarkably the production of tryptophan in a bench-scale fermentor. 5.5 g of tryptophan was accumulated per litre of culture medium at 24th hr in batch. The productivity was 0.229 g/l/hr. This productivity is the highest among those ever reported by other workers. The recombinant plasmid (Tcr Trp+ I-) used was completely stable in each run when tetracycline was added by 10 g/l into the medium. |
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