PGRL1 Participates in Iron-induced Remodeling of the Photosynthetic Apparatus and in Energy Metabolism in Chlamydomonas reinhardtii |
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| Authors: | Dimitris Petroutsos Aimee M. Terauchi Andreas Busch Ingrid Hirschmann Sabeeha S. Merchant Giovanni Finazzi Michael Hippler |
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| Affiliation: | From the ‡Institute of Plant Biochemistry and Biotechnology, University of Münster, Hindenburgplatz 55, 48143 Münster, Germany, ;the §Department of Chemistry and Biochemistry and Molecular Biology Institute, UCLA, Los Angeles, California 90095-1569, and ;the ¶UMR 7141 CNRS UPMC Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France |
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| Abstract: | PGRL1 RNA and protein levels are increased in iron-deficient Chlamydomonas reinhardtii cells. In an RNAi strain, which accumulates lower PGRL1 levels in both iron-replete and -starved conditions, the photosynthetic electron transfer rate is decreased, respiratory capacity in iron-sufficient conditions is increased, and the efficiency of cyclic electron transfer under iron-deprivation is diminished. Pgrl1-kd cells exhibit iron deficiency symptoms at higher iron concentrations than wild-type cells, although the cells are not more depleted in cellular iron relative to wild-type cells as measured by mass spectrometry. Thiol-trapping experiments indicate iron-dependent and redox-induced conformational changes in PGRL1 that may provide a link between iron metabolism and the partitioning of photosynthetic electron transfer between linear and cyclic flow. We propose, therefore, that PGRL1 in C. reinhardtii may possess a dual function in the chloroplast; that is, iron sensing and modulation of electron transfer. |
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