Heterogenous distribution of ferroportin-containing neurons in mouse brain |
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Authors: | Michael W Boserup Jacek Lichota David Haile Torben Moos |
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Institution: | (1) Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220 Aalborg East, Denmark;(2) South Texas Veterans Health Care System, San Antonio, TX, USA; |
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Abstract: | Iron is crucial for a variety of cellular functions in neuronal cells. Neuronal iron uptake is reflected in a robust and consistent
expression of transferrin receptors and divalent metal transporter 1 (DMT 1). Conversely, the mechanisms by which neurons
neutralize and possibly excrete iron are less clear. Studies indicate that neurons express ferroportin which could reflect
a mechanism for iron export. We mapped the distribution of ferroportin in the adult mouse brain using an antibody prepared
from a peptide representing amino acid sequences 223–303 of mouse ferroportin. The antibody specifically detected ferroportin
in brain homogenates, whereas homogenates of cultured endothelial cells were devoid of immunoreactivity. In brain sections,
ferroportin was confined to neuronal cell bodies and peripheral processes of cerebral cortex, hippocampus, thalamus, brain
stem, and cerebellum. In brain stem ferroportin-labeling was particularly high in neurons of cranial nerve nuclei and reticular
formation. Ferroportin was hardly detectable in striatum, pallidum, or hypothalamus. Among non-neuronal cells, ferroportin
was detected in oligodendrocytes and choroid plexus epithelial cells. A comparison with previous studies on the distribution
of transferrin receptors in neurons shows that many neuronal pools coincide with those expressing ferroportin. The data therefore
indicate that neuronal iron homeostasis consists of a delicate balance between transferrin receptor-mediated uptake of iron-transferrin
and ferroportin-related iron excretion. The findings also suggest a particular high turnover of iron in neuronal regions,
such as habenula, hippocampus, reticular formation and cerebellum, as several neurons in these regions exhibit a prominent
co-expression of transferrin receptors and ferroportin. |
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