Forskolin as a novel lipolytic agent |
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Authors: | R Ho Q H Shi |
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Institution: | Department of Biochemistry University of Miami School of Medicine P.O. Box 016129, Miami, Florida 33101 USA |
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Abstract: | Forskolin is a novel lipolytic agent which elevates cAMP and FFA release in rat adipocytes in a manner different from existing lipolytic factors. This effect of Forskolin is potentiated by all lipolytic hormones tested, i.e. epinephrine, ACTH, and glucagon and is also reversible. The same batch of adipocytes can be repeatedly stimulated after washing. The effective concentration of Forskolin is in the micromolar range. Its action is due to an activation of cAMP synthesis by adenylate cyclase. There is no effect on cAMP hydrolysis. In contrast to stimulation by lipolytic hormones, Forskolin-activated membrane adenylate cyclase was not further stimulated by GPP(NH)P. These results suggest that Forskolin may be a useful analytical agent in the study of adenylate cyclase mediated function in intact adipocytes. |
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Keywords: | cAMP adenosine cyclic 3′ 5′-monophosphate GPP(NH)P 5′-guanylimidodiphosphate FFA free fatty acids cAMP-PDE cAMP-phosphodiesterase |
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