Crystal structure of casein kinase-1, a phosphate-directed protein kinase. |
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Authors: | R M Xu G Carmel R M Sweet J Kuret and X Cheng |
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Institution: | W.M. Keck Structural Biology Laboratory, Cold Spring Harbor, NY 11724. |
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Abstract: | The structure of a truncated variant of casein kinase-1 from Schizosaccharomyces pombe, has been determined in complex with MgATP at 2.0 A resolution. The model resembles the 'closed', ATP-bound conformations of the cyclin-dependent kinase 2 and the cAMP-dependent protein kinase, with clear differences in the structure of surface loops that impart unique features to casein kinase-1. The structure is of unphosphorylated, active conformation of casein kinase-1 and the peptide-binding site is fully accessible to substrate. |
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