Nanosecond dynamics of charged fluorescent probes at the polar interface of a membrane phospholipid bilayer |
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Authors: | A P Demchenko N V Shcherbatska |
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Institution: | The Palladin Institute of Biochemistry, Academy of Sciences of the Ukrainian SSR, 252030 Kiev, U.S.S.R. |
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Abstract: | Molecular relaxation fluorescence methods were applied to analyze the nature and characteristic times of motions of amphiphilic molecules absorbed in the polar region of a phospholipid bilayer. The fluorescence probes 2-toluidinonaphthalene-6-sulfonate and 1-anilinonaphthalene-8-sulfonate in egg phosphatidylcholine vesicles were studied. The methods of edge excitation fluorescence red shifts, nanosecond time-resolved spectroscopy, fluorescence quenching by hydrophilic and hydrophobic quenchers and emission wavelength dependence of polarization were used. The structural (dipolar) relaxation is shown to be a very rapid (subnanosecond) process. The observed nanosecond phenomena are related to translational movement of the chromophore itself towards a more polar environment and its rotation. The polar surface area of the phospholipid membrane appears to be a highly mobile liquid-like system. |
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Keywords: | 1 8-ANS 1-anilinonaphthalene-8-sulfonate 2 6-TNS 2-toluidinonaphthalene-6-sulfonate Phospholipid membrane Amphiphilic molecule Fluorescent probe Red - edge excitation fluorescence shift Time-resolved spectroscopy Subnanosecond mobility Nanosecond mobility |
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