Improved green fluorescent protein as a fast reporter of gene expression in plant cells |
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| Authors: | Huang Yue Xu Xin-Ping Li Bao-Jian |
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| Affiliation: | 1. Biotechnology Research Center, Zhongshan University, Guang Zhou, 510275, P.R. China
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| Abstract: | An improved green fluorescent protein (GFP), S65TGFP, has new properties that make itself more suitable as a reporter of gene expression. The coding sequence for S65TGFP was placed under the control of the rice actin1 (Act1) promoter in pAct1-S65TGFP reconstruction. We transformed pAct1-S65TGFP into rice callus cells by particle bombardment and bright green fluorescent dots could be seen after 6-8 hours. |
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