Sexual difference in properties of 5 alpha-reductase in microsome of rat submandibular glands |
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| Authors: | S Koshika M Hosaka M Tarui M Furuyama Y Nakayama |
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| Institution: | Department of Biochemistry, Kanagawa Dental College, Yokosuka, Japan. |
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| Abstract: | The properties of 5 alpha-reductase activity in the submandibular glands of rats were investigated using microsomal fraction in the presence of NADPH, and we found a sexual difference in these properties. The formation of 5 alpha-dihydrotestosterone and 5 alpha-androstane-3 alpha, 17 beta-diol from testosterone demonstrated 5 alpha-reductase in the rat microsomal fraction of this tissue sample. Apparent michaelis constants (Km) of the 5 alpha-reductase activity for testosterone was 1.02 x 10(-6) M for the female tissue. Microsomal fraction of submandibular glands of male rats had two Kms and were determined as 1.12 x 10(-6) M and 1.01 x 10(-5) M. The lower Km of 5 alpha-reductase for male rats was similar to for the females. |
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