| EPSTEIN-BARR病毒血清抗体阳性健康人外周血体外培养“自发性”转化的研究 |
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| 引用本文： | 黄玫玲,冯凯涛,姚庆云.EPSTEIN-BARR病毒血清抗体阳性健康人外周血体外培养“自发性”转化的研究[J].病毒学报,1986(2). |
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| 作者姓名： | 黄玫玲 冯凯涛 姚庆云 |
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| 作者单位： | 中山医科大学肿瘤研究所
(黄玫玲,冯凯涛),中山医科大学肿瘤研究所(姚庆云) |
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| 摘 要： | 对35名Epstein-Barr病毒血清抗体阳性健康人的外周血淋巴细胞进行了体外培养,观察B淋巴细胞感染了EB病毒后所导致“自发性”转化的情况。由于在培养基中加入了免疫抑制剂环胞菌素A,使“自发性”转化的发生率由26.7％提高到74.3％,说明了在血清抗体阳性健康人的外周血液中,EB病毒感染的B淋巴细胞的数量远比既往文献报道的高。
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| 关 键 词： | Epstein-Barr病毒 自发性转化 环胞菌素A |
"SPONTANEOUS TRANSFORMATION" IN PERIPHERAL BLOOD OF HEALTHY EPSTEIN-BARR VIRUS SEROPOSITIVE INDIVIDUALS |
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| Huang Meiling Feng Kaitao Yao Qingyun."SPONTANEOUS TRANSFORMATION" IN PERIPHERAL BLOOD OF HEALTHY EPSTEIN-BARR VIRUS SEROPOSITIVE INDIVIDUALS[J].Chinese Journal of Virology,1986(2). |
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| Authors: | Huang Meiling Feng Kaitao Yao Qingyun |
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| Institution: | Cancer Institute Sun yat-sen University of Medical Sciences |
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| Abstract: | A panel of 35 healthy seropositive donors have been studied for the detection of Epstein-Barr virus ( EBV ) infected B cells in their periperal blood Cultures were set up and maintained in medium containing the immunosup-pressive drug cyclosporin A ( CSA at 0. lug/ml) .In the majority of cases,parallel cultures were also set up in medium without CSA.The cells were cultured for 6-8 weeks to ascertain the incidence of EBV-induced "spontaneous trasformation" into lymphoblastoid cell lines. Addition of CSA increased the positive rate from 26.7% to 74.3%. EBV infected B cells in the blood of healthy seropostive individuals were detected more often in this study than in previous reports,spontaneous transformation is more imporant for the study of EBV carrier state than test for antibody. |
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| Keywords: | Epstein-Barr virus Spontaneous transformation Cyclosporin A |
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