Crystallization and preliminary X-ray studies of psophocarpin B1, a chymotrypsin inhibitor from winged bean seeds |
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| Authors: | J K Dattagupta C Chakrabarti A Podder S K Dutta M Singh |
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| Institution: | Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Bidhan Nagar, Calcutta, India. |
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| Abstract: | Psophocarpin B1 is a 20,000 Mr protein of winged bean (Psophocarpus tetragonolobus) seeds having chymotrypsin inhibitory activity. Single crystals of this protein suitable for X-ray crystallographic studies have been obtained by the vapour diffusion method using ammonium sulphate. The crystals are hexagonal, space group P6(4)22 or P6(2)22, cell dimensions a = b = 61 A, c = 210 A. They are stable to irradiation with X-rays and diffract to at least 2.6 A resolution. |
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