| 精制地鼠肾细胞狂犬病疫苗纯化工艺的建立 |
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| 引用本文： | 吕宏亮,宋继萍,邹勇,王玉琳,周书全.精制地鼠肾细胞狂犬病疫苗纯化工艺的建立[J].生物技术通讯,2001,12(3):205-207. |
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| 作者姓名： | 吕宏亮 宋继萍 邹勇 王玉琳 周书全 |
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| 作者单位： | 1. 卫生部兰州生物制品研究所;中国预防医学科学院病毒学研究所
2. 卫生部兰州生物制品研究所 |
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| 摘 要： | 分别用Srpharose4B、Sephacryl-200HR和Sepharose6-Fastflow柱色谱进行地鼠肾细胞狂犬病疫苗浓缩原液纯化试验,试制精制地鼠肾细胞狂犬病疫苗.对疫苗的残余牛血清含量、效力、收获率和安全性进行检测,优化和建立了精制地鼠肾细胞狂犬病疫苗纯化工艺.
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| 关 键 词： | 狂犬病疫苗 纯化 安全 效力 收获率 |
| 修稿时间： | 2000年10月30 |
A procedure of purification for preparing purified primary hamster kidney cell rabies vaccine |
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| LV Hong-Liang,SONG Ji-Ping,ZOU Yong,WANG Yu-Lin,ZHOU Shu-Quan.A procedure of purification for preparing purified primary hamster kidney cell rabies vaccine[J].Letters in Biotechnology,2001,12(3):205-207. |
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| Authors: | LV Hong-Liang SONG Ji-Ping ZOU Yong WANG Yu-Lin ZHOU Shu-Quan |
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| Abstract: | To prepare purified primary hamster kidney cell rabies vaccine (PPHKCRV), Sepharose4B,Sephacryl-200HR,Sepharose 6-Fast Flow chromatography were used respectively in this experiment. By checking residual calf serum contents, safety, potency and harvest recovery of purifying rabies vaccine, the methods mentioned above were screened. A purification procedure that was suitable to large scale production of purified primary hamster kidney cell rabies vaccine was established. |
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| Keywords: | rabies vaccine purification safety potency recovery |
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