Different Assessment Techniques of Leaf Blotch (Rhynchosporium secalis (Oud.) J. Davis) in Winter Barley after Artificial Inoculation

Seven winter barley cultivars expressing different field resistance against leaf blotch (Rhynchosporium secalis) and 60 doubled haploid (DH) lines descending from and F₁ hybrid resistant x susceptible were used to develop a test for the quantitative determination of disease severity. Differences in the level of resistance could be measured very precisely after artificial infection of seedlings at the 4-leaf-stage and subseqent incubation at 7 C. The protein of six isolates was usedf, or antiserum production which was subsequently supplied for serological testing (Enzyme-Linked-Immunosorbent-Assay) of infected leaves. The results of visible scoring of the seedlings was significantly correlated with measuring with the ELISA reader. The, results of the seedling test also corresponded to field scores after natural infection.