| 南阳牛DGAT2基因PCR-RFLP多态性及其与生长性状相关性研究 |
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| 引用本文： | 张争锋,陈宏,李秋玲,雷初朝,张春雷,王新庄,王居强,王轶敏.南阳牛DGAT2基因PCR-RFLP多态性及其与生长性状相关性研究[J].遗传,2007,29(8):945-945―950. |
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| 作者姓名： | 张争锋 陈宏 李秋玲 雷初朝 张春雷 王新庄 王居强 王轶敏 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100;2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116;3. 河南省肉牛工程技术研究中心, 郑州 450003;4. 山东省计算中心, 济南 250014;5. 山东省农业科学院奶牛研究中心, 济南 250100 ; |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家科技支撑计划，河南省高校杰出科研创新人才工程项目，西北农林科技大学校科研和教改项目 |
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| 摘 要： | 以131头纯种南阳牛为研究材料, 利用PCR－RFLPs对二酰甘油酰基转移酶2(DGAT2)基因的第6内含子和第7内含子的多态性及其与生长发育的相关性进行了分析。结果表明: 南阳牛在该位点分别检测到两种等位基因A/B和N/M, 频率分别为0.875/0.125和0.971/0.029。A和N等位基因是群体中的优势等位基因。该基因内含子6对南阳牛6月龄的体高、2岁体重、6月龄到两岁的胸围和体斜长都有显著的影响, 内含子6的AA基因型的6月龄的体高比杂合型高3.8 %, 两岁体重高3.9%, 6月龄到两岁的胸围分别高3.8%、3.4%、3.7%、4.3%; 6月龄到两岁的体斜长分别高3.8%、3.6%、3.8%、3.1%。内含子7对18月龄和两岁的坐骨端宽有极显著的影响(P<0.01); 对两岁的胸围有显著的影响(P<0.05)。
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| 关 键 词： | DGAT2 南阳牛 PCR-RFLP 生长性状 |
| 文章编号： | 10.1360/yc-007-0945 |
| 收稿时间： | 2007-01-09 |
| 修稿时间： | 2007-01-09 |
Polymorphisms of DGAT2 gene and its associations with several growth traits in Nanyang cattle |
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| ZHANG Zheng-Feng,CHEN Hong,LI Qiu-Ling,LEI Chu-Zhao,ZHANG Chun-Lei,WANG Xin-Zhuang,WANG Ju-Qiang,WANG Yi-Min.Polymorphisms of DGAT2 gene and its associations with several growth traits in Nanyang cattle[J].Hereditas,2007,29(8):945-945―950. |
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| Authors: | ZHANG Zheng-Feng CHEN Hong LI Qiu-Ling LEI Chu-Zhao ZHANG Chun-Lei WANG Xin-Zhuang WANG Ju-Qiang WANG Yi-Min |
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| Institution: | 1. College of Animal Science and Technolog, Shaanxi Key Laboratory of Molecular Biology for Agricultue, Northwest A&F University, Yangling 712100, China;2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China;3. Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003, China;4. Shandong Computer Science Center, Ji’nan 250014 China;5. Dairy Cattle Rearch Center, Shandong Academy of Agricultural Science, Ji’nan 250100, China ; |
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| Abstract: | The associations between SNPs in intron 6 and intron 7 of the bovine diacylglycerolacyltransferase (DGAT2) gene and growth traits in purebred Nanyang cattle (n=131) were reported in this study. Alleles detected in intron 6 were allele A and B with frequencies of 0.875 and 0.125,respectively; alleles detected in intron 7 were allele N and M with frequencies of 0.968 and 0.032,respectively. The animals with genotype AA in intron 6 showed 3.8% increases in body height(P < 0.01),3.9% increases in body weight (P < 0.05),3.8% (P < 0.05),3.6% (P < 0.05),3.8% (P < 0.01) and 3.1%( P < 0.01) increase in body length at age six-month to two-year compared with genotype AB,respectively. They also showed 3.8 % (P < 0.05),3.4 % ( P < 0.01),3.7% (P < 0.01) and 4.3% (P < 0.01) increases in heart girth at age six-month to two-year respectively. Animals with the NM genotype show significantly higher average heart girth at age two years (P < 0.05),and Hucklebone width (P < 0.01) at age eighteen-month (P < 0.01) and two-year (P < 0.01) compared with animals with the genotype NN,respectively. |
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| Keywords: | DGAT2 PCR-RFLP |
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