Oxidation of lysozyme by iodine: isolation of an inactive product and its conversion to an oxindolealanine-lysozyme |
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Authors: | T Imoto F J Hartdegen J A Rupley |
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Affiliation: | Department of Chemistry University of Arizona Tucson, Ariz. 85721, U.S.A. |
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Abstract: | Reaction of lysozyme with a half-molar amount of iodine at pH 5.5 gives, in 50% yield (based on iodine), an enzymatically-inactive singly-oxidized derivative. This intermediate can be converted to an oxindole-lysozyme. Analysis showed no modification other than two-electron oxidation of one tryptophan. Substrate analogs prevent the oxidative inactivation. Reaction of crystalline lysozyme with iodine gives the same inactive product found in solution. |
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Keywords: | β(1 → 4)-linked oligomer of N-aoetylgluoosamine |
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