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Microsatellite-based parentage control in cattle |
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| Authors: | M-L Glowatzki-Mullis C Gaillard G Wigger R Fries |
| Affiliation: | Institute for Animal Breeding, University of Berne, Bremgartenstrasse 109A, CH-3012 Berne, Switzerland;Department of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zurich, Switzerland |
| Abstract: | As a new approach to parentage control we developed two multiplex coamplification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite polymorphisms were visualized by automated fluorescence detection on the Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis software. Allele frequency data were determined from 238 animals. Thirty-five bovine parentage control cases not solvable by conventional blood typing could be solved. |
| Keywords: | parentage control microsatellite/STR typing cattle |