Overexpression of <Emphasis Type="Italic">odc</Emphasis> (ornithine decarboxylase) in <Emphasis Type="Italic">Datura innoxia</Emphasis> enhances the yield of scopolamine |
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Authors: | Alka Singh Niraj K Nirala Sandip Das Alka Narula M V Rajam P S Srivastava |
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Institution: | (1) Department of Biotechnology, Hamdard University, New Delhi, 110062, India;(2) Present address: Program in gene Function and Expression, Umass Medical School, Worcester, 01605, USA;(3) Department of Genetics, Delhi University, South Campus, New Delhi, 110021, India; |
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Abstract: | Scopolamine is widely used for its anticholinergic properties. Because of higher physiological activity and less side effects
the world demand of scopolamine is estimated to be ten times greater than other anticholinergic agents, hyoscyamine and atropine.
Since natural production is limited, alternatives are required to boost the production. We report the introduction of mouse
odc gene of polyamine biosynthesis pathway which is also the primary pathway of tropane alkaloids in Datura innoxia. Polyamines, mainly putrescine, serve as the common metabolite for tropane alkaloids and nicotine. We have overexpressed
odc gene to modulate the metabolic flux downstream and eventually achieved higher accumulation of scopolamine in transgenic plants.
Among six independent transformed lines one line (O10) produced scopolamine (0.258 μg/g dry weight) almost six times higher
than that produced by control plants (0.042 μg/g DW). To our knowledge, this is the first report of odc overexpression in D. innoxia leading to higher scopolamine yield. |
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