<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated genetic transformation of economically important oilseed rape cultivars |
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Authors: | Eva Boszoradova Jana Libantova Ildiko Matusikova Zuzana Poloniova Martin Jopcik Maria Berenyi Jana Moravcikova |
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Institution: | (1) Institute of Plant Genetics and Biotechnology, SAS, Akademicka 2, P.O.Box 39A, 95007 Nitra, Slovakia;(2) AIT Austrian Institute of Technology GmbH, Konrad-Lorenz Str. 24, 3430 Tulln, Austria; |
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Abstract: | Oilseed rape is considered relatively recalcitrant for genetic modification. This work was performed to establish conditions
for effective transformation and regeneration of commercially used cultivars Campino, Haydn, Heros, Hunter and Topas (Brassica napus L.). Cotyledonary petioles and hypocotyls (obtained from the seedlings grown under dark conditions) as a source of explants
were used. Our experiments revealed that a lower selection pressure in combination with postponing of selection by 14 days
after co-cultivation resulted in recovery of transgenic plants from all cultivars. Transformants were obtained with efficiency
from 1 to 5.5%. Histochemically GUS-positive plants were analysed by PCR using primers corresponding to the internal fragments
of gus and nptII genes. The transgenic nature was confirmed by Southern blot analyses using a specific nptII probe. This work enriches the list of oilseed rape cultivars available for genetic modification. |
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