Modified Cetyltrimethylammonium bromide method improves robustness and versatility: The benchmark for plant RNA extraction |
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Authors: | Elizabeth J White Mauritz Venter Dr Nicholas F Hiten Johan T Burger |
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Institution: | 1. Department of Genetics, Stellenbosch University, Matieland, South Africa;2. Institute for Plant Biotechnology, Department of Genetics, Stellenbosch University, Matieland, South Africa |
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Abstract: | A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness and versatility, we have improved the cetyltrimethylammonium bromide (CTAB) method and isolated high-quality RNA from 16 different plant species. The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in β-mercaptoethanol concentration (to 3%) resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types. |
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Keywords: | Cetyltrimethylammonium bromide Plant leaf RNA RT-PCR |
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