Biphasic mini-reactor for characterization of biocatalyst performance |
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Authors: | Anne van den Wittenboer Thomas Schmidt Pia Müller Marion B Ansorge-Schumacher Lasse Greiner Dr |
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Institution: | 1. Department of Biotechnology, RWTH Aachen University, Aachen, Germany;2. Institut für Technische und Makromolekulare Chemie, RWTH Aachen University, Aachen, Germany;3. Institute of Chemistry, Enzyme Technology, TU Berlin, Berlin, Germany |
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Abstract: | Biphasic reaction media are extending the scope of technical biocatalysis. Thorough investigation of the factors affecting catalyst performance under these conditions is of key importance for the successful implementation of catalytic processes. Here, we present a reactor setup suitable for comprehensive systematic characterization and optimization of biocatalyzed reactions in biphasic systems with distinct phases. It is distinguished by small volumes allowing reproducible experimentation with minimum amounts of solvent and catalyst. The interfacial area is kept constant and independent stirring of both phases is allowed in order to minimize superimposing effects. Evaporation of low-volatile organic solvents is prevented by use of airtight construction. The broad applicability of this mini-reactor is demonstrated with regard to determination of mass transfer, enzyme productivity, and enzyme stability in both batch and continuous mode. |
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Keywords: | Biocatalysis Biphasic media Mini-reactor |
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