Soft X-ray tomography: virtual sculptures from cell cultures
Institution:
2. Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, United Kingdom
Abstract:
(a) Cells are taken from their culture media, mounted on grids or in capillaries, and cryopreserved. Tomographic data are collected in a soft X-ray microscope with the cells maintained at cryogenic temperature. (b) A single slice through a 3D tomographic reconstruction of an HT1080 fibrosarcoma cell. The slice comprises tomographic reconstructions from two adjacent fields of view computationally stitched together. (c) 3D volume rendering of the tomographic reconstruction shown in (b) after segmentation guided by linear absorption coefficients (LAC) and organelle morphology. Key: Red; Nucleus, Turquoise; Mitochondria, Blue; Lipids, Gray; cell membrane. Scale bar = 1.5 μm.
