Cytotoxic T-cell responses to H-Y:Ir genes and associative antigens map inH-2

The secondary cytotoxic responses to the male-specific antigen (H-Y) in mice showH-2 restriction so that the cytotoxic female cell must share the K- and/or D-end antigen with the male target cells. The association with the K and/or D end varies with differentH-2 haplotypes,e.g., H-2 ^b cytotoxic cells require the H-2D^b antigen(s) on the target cells, while cytotoxic cells fromH-2 ^b/H-2 ^d F₁ mice sensitized toH-2 ^d male cells kill only male targets having H-2K^d antigen(s). This association of H-Y with appropriate K/D antigens seems to be needed also in the induction of the cytotoxic response. Of the independent haplotypes, onlyH-2 ^b strains are capable of making secondary anti-H-Y responses and this trait seems to be dominant,i.e., the F₁ strains with oneH-2 ^b parent are able to produce anti-H-Y cytotoxic cells against both theH-2 ^b parent and the nonresponder parent. The mating of the two nonresponder strains may produce F₁ mice which are responders, thus suggestingIr gene complementation. Mapping data indicates that at least one of these complementary genes is located in theI-C region fork/s complementation.