Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid. |
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| Authors: | H Tanimura M Maeda T Fukazawa M Sekine and T Hata |
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| Institution: | Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan. |
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| Abstract: | A general and practical synthetic method of oligoribonucleotides (10-20 mers) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl (Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5'-hydroxyls and tetrahydropyranyl (Thp) group was used for the 2'-hydroxyl protecting groups. In addition, suitable acyl groups were introduced for the protection of the lactam functions of guanine and uracil moieties. |
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