Mass production of HzSNPV baculoviruses in immobilized<Emphasis Type="Italic">Heliothis zea</Emphasis> (HzAM1) insect cell culture期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Mass production of HzSNPV baculoviruses in immobilizedHeliothis zea (HzAM1) insect cell culture

Authors:	Jeong?Hwa?Son,Rainer?Buchholz,Sung-Koo?Kim author-information" > author-information__contact u-icon-before" > mailto:skkim@pknu.ac.kr" title=" skkim@pknu.ac.kr" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Department of Biotechnology & Bioengineering, Pukyong National University, 608-737 Busan, Korea;(2) Department of Bioprocess Engineering, Friedrich-Alexander University, Erlangen-Nuremberg, Germany

Abstract:	Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMI cell density was 7.5×10⁷ cells/mL in the microspheres. After infection of the immobilized cells byHeliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was 2.87×10¹⁰ PIBs/mL in the microspheres.

Keywords:	HzAM1 insect cell HzSNPV NaCS PDADMAC PIBs
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