Pseudomonas mutants able to accumulate phenolic and catecholic 9,10-secosteroids from bile acids |
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| Authors: | J A Ide R J Park R A Leppik N W Dunn |
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| Institution: | (1) Biotechnology, University of New South Wales, 2033 Kensington, NSW, Australia;(2) Meat Research Laboratory, CSIRO Division of Food Research, 4170 Cannon Hill, Queensland, Australia |
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| Abstract: | Summary An NTG induced mutant of a bile acid utilizing strain of Pseudomonas putida was isolated which was blocked in the steroid catabolic pathway. This mutant (PS5-7) was able to accumulate both phenolic and catecholic secosteroid products from cholic acid. Using a transposing system containing the kanamycin resistance transposon Tn5, mutants of PS5-7 were isolated which produced only the phenolic secosteroid. One of these mutants (PS5-25) was able to produce close to theoretical yields of various phenolic secosteroids from corresponding bile acids. |
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