Glutamine uptake by rat renal basolateral membrane vesicles |
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| Authors: | Robert A. Reynolds Hanna Wald Stanton Segal |
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| Affiliation: | (1) Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Blvd., 19104 Philadelphia, PA, USA;(2) Departments of Pediatrics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA;(3) Present address: Nephrological Service, Hadassah University Hospital, Ein Kerem, Jerusalem, Israel |
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| Abstract: | The presence of a sodium-dependent, saturable uptake process is described in basolateral membranes of rat renal cortex for L-glutamine. Concentration-dependence studies indicate the presence of multiple transport systems withKm1 of 0.032 mM and V1 of 0.028 nmol/mg of protein per min, andKm2 of 17.6 mM and V2 of 17.6 nmol/mg of protein per min. Lysine completely inhibits the high-affinity, low-capacityKm system and partially inhibits the low-affinity, high-capacity system. Cystine and other dibasic amino acids also affect glutamine uptake. |
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